Agus Sunadi Putra earns a Doctor of Medicine degree after discovering the potential of Euphorbia Milii leaves to speed up the healing process of burns.

Located in the Lt III Postgraduate meeting room, a Doctoral Promotion exam was held with promovendus candidate I Made Agus Sunadi Putra, S.Sc., M.Biomed., Apt with the dissertation title "Ethyl Acetate Extract of Euphorbia Milii Leaves Reduces Malondialdehyde Levels and Number of Bacterial Colonies Pseudomonas aeruginosa and Increasing Vascular Endothelial Growth Factor Levels and Number of Fibroblasts in Wistar Rats with Pseudomonas Aeruginosa Infected Burns".

Wounds with infections often found in hospitals are called nosocomial infections that occur in patients who are hospitalized for at least 72 hours. Infection is often caused by bacteria, including Pseudomonas aeruginosa. Currently, therapy with antibiotics is already able to treat burns properly. However, with the frequent occurrence of antibiotic resistance, other solutions need to be found, such as the use of natural materials, one of which is from Euphorbia milii leaves. Wound healing is a dynamic and complex biological process, going through several phases, namely coagulation, inflammation, proliferation, remodeling. This study aims to prove the effect of Euphorbia milii leaf extract in the wound healing process seen from the decrease in the amount of malondialdehyde, decrease in the number of Pseudomonas aeruginosa bacterial colonies, increase in the amount of Vacular Endothelial Growth Factor (VEGF), and increase in the number of fibroblasts at the inflammatory stage.

Administration of ethyl acetate extract of Euphorbia milii leaves to the burns of male rats Raatus norvegicus Wistar strain infected with Pseudomonas aeruginosa resulted in a decrease in malondialdehyde levels at the beginning of the maturation process which was seen on day 21 and lower than the control. In relation to the effect on Pseudomonas aeruginosa bacteria, the ethyl acetate extract of Euphorbia milii leaves resulted in a decrease in the number of bacterial colonies at the start of the maturation process but not significantly different compared to the control. The ethyl acetate extract of Euphorbia milii leaves also resulted in an increase in VEGF levels and the number of fibroblasts in the proliferative phase which were seen on day 14 and had higher levels compared to controls.

The novelty of this research is the pathogenesis of burns infected with the bacterium Pseudomonas aeruginosa, where the topical administration of Euphorbia milii leaf extract can accelerate the healing process of burns by reducing oxidative stress which can be observed through decreasing levels of Malondialdehyde, increasing vascularization marked by increasing levels of VEGF. and also increased the number of fibroblasts in Wistar rats with burns infected with Pseudomonas aeruginosa.

The implication of this study is that the content of flavonoids, tannins, saponins from Euphorbia milii leaves work in accelerating wound healing by preventing oxidative damage, increasing angiogenesis by increasing VEGF, increasing the number of fibroblasts and reducing the number of Pseudomonas aeruginosa bacteria. This study proves that the ethyl acetate extract of Euphorbia milii leaves plays a role in healing burns infected with the Pseudomonas aeruginosa bacteria.

The exam was led by Deputy Dean of General Affairs and Finance, Faculty of Medicine, Udayana University, Dr. dr. I Made Sudarmaja, M.Kes., with a team of examiners:

1. drh. I Nyoman Mantik Astawa, Ph.D (Promoter)

2. Prof. Dr. dr. I Dewa Made Sukrama, M.Si., Sp.MK (K)

(Co-promoter I)

3. Dr. dr. I Made Jawi, M.Kes (Co-promoter II)

4. Dr. dr. I Nyoman Mangku Karmaya, M.Repro., PA (K)

5. Prof. Dr. dr. I Made Bakta, Sp.PD-KHOM

6. Dr. dr. I Wayan Putu Sutirta Yasa, M.Si

7.Dr. dr. I Made Sudarmaja, M. Kes

8. Dr. drh. I Wayan Suardana, M.Sc

9.Dr. dr. I Nyoman Wande, S.Ked.,Sp.PK(K)

10.Dr. dr. Sianny Herawati, Sp. PK(K)

 

While academic invitations are:

1. dr. Ni Nengah Dwi Fatmawati, S.Ked., Sp.MK(K).,Ph.D

2.Dr. dr. Made Agus Hendrayana, M.Ked

3.Dr. drh. I Made Subrata, M. Erg

4.Dr. dr. Dewa Ayu Agus Sri Laksemi, M.Sc

5. Desak Ketut Ernawati, S.Si, Apt., P.G.Pharm., M.Pharm., Ph.D.

 

In this exam, Dr. I Made Agus Sunadi Putra, S.Sc., M.Biomed., Apt., was declared the 365th Doctoral Graduate of the Doctoral Study Program in Medical Sciences, Faculty of Medicine, Udayana University with the title of VERY SATISFACTORY.