Study of Oil Water Ratio and Concentration of Resting Cells of Bacterial Isolates SBJ8 on Activity Testing Biodesulfurization Dibenzothiophene in Oil Model Tetradecane



By : I Gd Krisna Putra Pratama

Email :

Faculties : Fakultas Teknologi Pertanian

Department : S1 Teknologi Industri Pertanian

This study aims to determined the best ratio of oil / water and concentration of resting cells and time course pattern resting cells of bacteria isolate SBJ8 to be known the biodesulfurization capability in two-phase media using media Mineral salt sulfur free-tetradecane (MSSF TD). Oil/water ratio used in this study are 1:1, 1:2, and 1:4 in combination with cell concentration by OD660 10, 20, and 30. The research was conducted using 200 mg l1 dibenzothiophene as a source of sulfur dissolved in tetradecane as a model of petroleum (oil phase). Isolates were incubated for 24 hour at 37C and the residue of dibenzothiophene analyzed by gas cromathography mass selective (GC MS). Testing the degradation of resting cells is accomplished by two replications. The results showed that the ratio of oil water 1:4 and cell concentration OD660 30 shows the results of the highest degradation of 84.84% and the time course for 24 hours resting cells show degradation rate dibenzothiophene up to 81.84%.

Keyword : Biodesulfurization, Dibenzothiophene, Tetradecane, Resting Cell

Download : File 1 File 2 File 3