Study of Oil Water Ratio and Concentration of Resting Cells of Bacterial Isolates SBJ8 on Activity Testing Biodesulfurization Dibenzothiophene in Oil Model Tetradecane
By : I Gd Krisna Putra Pratama
Faculties : Fakultas Teknologi Pertanian
Department : S1 Teknologi Industri Pertanian
This study aims to determined the best ratio of oil / water and concentration of resting cells and time course pattern resting cells of bacteria isolate SBJ8 to be known the biodesulfurization capability in two-phase media using media Mineral salt sulfur free-tetradecane (MSSF – TD). Oil/water ratio used in this study are 1:1, 1:2, and 1:4 in combination with cell concentration by OD660 10, 20, and 30. The research was conducted using 200 mg · l–1 dibenzothiophene as a source of sulfur dissolved in tetradecane as a model of petroleum (oil phase). Isolates were incubated for 24 hour at 37°C and the residue of dibenzothiophene analyzed by gas cromathography mass selective (GC – MS). Testing the degradation of resting cells is accomplished by two replications. The results showed that the ratio of oil water 1:4 and cell concentration OD660 30 shows the results of the highest degradation of 84.84% and the time course for 24 hours resting cells show degradation rate dibenzothiophene up to 81.84%.
Keyword : Biodesulfurization, Dibenzothiophene, Tetradecane, Resting Cell