THE EFFECT OF SUJI (PLEOMELE ANGUSTIFOLIA N.E. BROWN) LEAF ETHANOL EXTRACT ON THE VIABILITY OF LYMPHOCYTES IN PBMC CULTURES WERE EXPOSED TO H2O2 3 PERSEN

SKRIPSI

Abstract

By : Apolonia Berenika Badu

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Faculties : Fakultas Kedokteran

Department : PS. PENDIDIKAN DOKTER

Currently the dangerous effects of free radicals more increased by the presence of global warming and consumption of unhealthy foods, then we need a strategy to reduce or minimize these effects. One of the strategies is the need for a substance that can reduce oxidative effects of free radicals and can increase the body’s immune system to protect and improve the viability of lymphocytes. This study aim to know the effect of Suji (Pleomele angustifolia N.E. Brown) leaf ethanol extract on lymphocytes in PBMC cultures were exposed to H2O2 3%. The design of this study is purely experimental with randomized post test only control group design. The samples used were lymphocytes in PBMC cultures as much as 594 x 102 cells/ml. The independent variable in this study is the concentration of the Suji leaf ethanol extract, the dependent variable is the lymphocyte viability. Data were analyzed by One Way ANOVA followed by Post Hoc test. At a concentration of 0.00032 g/ml, after observation for 24 hours, the average number of lymphocyte viability is 94.62%. One Way ANOVA test obtain the results of the p value = 0.002 (p<0.05), which means that there is a statistically significant difference on the viability of lymphocytes of treatment between the groups studied. Results of Post Hoc analysis there are significant differences in P0 and P1, P1 and P2, P1 and P3, P1 and P4 (p<0.005). Suji leaf ethanol extract is effective in increasing the viability of the lymphocyte cell counts compared with the positive control. The concentration of 0.00032 is the most effective concentration. Further study is needed with the same lot number of samples for each treatment.

Keyword : leaf suji, lymphocytes, H2O2

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