THE EFFECT OF ETHANOL EXTRACT OF MAHKOTA DEWA FRUIT PULP (PHALERIA MACROCARPA) AGAINST VIABILITY OF LYMPHOCYTES CELLS IN PBMC CULTURES EXPOSED TO 3 PERCENT H2O2

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Abstract

By : Ni Wayan Devi Yulianti

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Faculties : Fakultas Kedokteran

Department : PS. PENDIDIKAN DOKTER

The immune system has a vital role for health. The immune system is necessary to maintain the integrity against danger posed by a variety of materials in the environment, one of them is free radicals. Antioxidant can reduce the reactivity of free radicals. Mahkota Dewa, especially the fruit pulp contains antioxidants, specifically flavonoids. This study aimed to determine the effect of ethanol extract of Mahkota Dewa Fruit Pulp (Phaleria Macrocarpa) against viability of lymphocytes cells in PBMC cultures exposed to 3% H2O2 The design of this study was purely experimental with patterns of Post Test Only Control Group Design. The samples used were lymphocytes in PBMC cultures as much as 816 X 102 cells/ml. The independent variable in this study was the concentration of the ethanol extract of Mahkota Dewa Fruit Pulp, the dependent variable is the viability of lymphocytes cells. Data were analyzed by One Way ANOVA followed by Post Hoc test. At a concentration of 0.00000196 g/ml (P4) after observation for 30 minutes, obtained the highest value of viability 92.58%. In One Way ANOVA obtain results p value = 0.001 (p <0.05), which means that there is a difference viability of lymphocyte cell between groups were examined. In the Post Hoc analysis results obtained are the most difference is statistically significant at P1 and P4 group (p = 0.001). Ethanol extract of Mahkota Dewa Fruit Pulp effective in increasing viability of lymphocyte cells compared to the positive control, especially in concentration 0.00000196 g/ml, where it was the most effective concentration. Further research is needed with the homogen number of samples in each grup and a lot more varied concentrations.

Keyword : Mahkota dewa fruit pulp, lymphocytes, H2O2

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