The Effect of Ethanol Extract of Juwet Fruit Skin (Syzygium Cumini) Against Viability of Lymphocyte Cells In PBMC Cultures Exposed to H2O2 3 percent

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Abstract

By : Luh Putu Diah Ratna Iswari Sudewa

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Faculties : Fakultas Kedokteran

Department : PS. PENDIDIKAN DOKTER

Humans seek to inhibit the effects of free radicals in the body so that the effect can be inhibited. One natural way to inhibit free radicals in the body is by eating foods that contain antioxidants, such as eating juwet fruits (Syzygium cumini). This study aimed to determine the effect of ethanol extract of the juwet fruit skin (Syzygium cumini) against viability of lymphocyte cells in PBMC cultures exposed to H2O2 3%. This study is a pure experiment with pattern Post Test Only Control Group Design. The samples used were lymphocytes in PBMC cultures as much as 766 x 102 cells/ml. The independent variable in this study is the concentration of the ethanol extract of the juwet fruit skin (Syzygium cumini) and as the dependent variable lymphocyte cells. Data were analyzed by One Way Anova followed by Post Hoc test. The concentration of 0.00002 g/ml (P4) after observation for 30 minutes, obtained the highest viability value of 88.74%. One Way Anova obtain results p value = 0.003 (p <0.05). That means, there is a statistically significant difference in lymphocyte cell viability of each group was studied. Based on the results of Post Hoc analysis obtained statistically significant differences in the highest at P4 and P1 (p =0.002). Ethanol extract of the juwet fruit skin is effective in improving the viability of lymphocytes compared with the positive control. The concentration of 0.00002 g/ml is more effective. Further research is needed with the same number of samples as well as the concentration of each group much more varied.

Keyword : juwet fruit skin, lymphocyte cells, H2O2

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