The Effect Of Ethanol Extract Of Bay Leaves (Eugenia Polyantha) Against Viability Of Lymphocyte Cells In PBMC Cultures Exposed to H2O2 3 Percent

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Abstract

By : Agus Dody Pranata Suadi Putra

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Faculties : Fakultas Kedokteran

Department : PS. PENDIDIKAN DOKTER

The development of science and technology caused destruction of the environment and change of lifestyle in human. Instant of lifestyle and stress that experienced by human potentially gained exposure to free radicals. Free radials can muffled using antioxidant. Bay were one of plant that contains antioxidant, especially flavonoid. This study aimed to determine the effect of ethanol extract of bay leaves (Eugenia polyantha) against viability of Lymphocyte Cells in PBMC cultures exposed to 3% H2O2. The design of this study is purely experimental with patterns of Post Test Only Control Group Design on venous blood samples by in vitro. The sample used in this study were lymphocytes in PBMC cultures as much as 808 x 102 cells / ml. The independent variable in this study was the ethanol extract of bay leaves (Eugenia polyantha). The dependent variable in this study were lymphocytes. Data were analyzed by One-Way ANOVA followed by Post Hoc test. After observation for 30 minutes, obtained the highest viability value in group P4 (86.07%). In One-Way ANOVA test obtained p value = 0.001 (p <0.05), which means that there is significant difference on the viability of lymphocytes in the experiment. In Post Hoc test obtained difference is statistically significant at P0 to P4, P1 to P3, and P1 to P4 group. Ethanol extract of bay leaves (Eugenia polyantha) effective in increasing the viability of the lymphocyte cell compared to positive control. The concentration of 0.0000054 g/ml were the most effective concentration. Further research is needed with the homogen number of sample and a lot more varied concentrations.

Keyword : ethanol extract of bay leaves, lymphocyte cells, H2O2

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