Sensitvity and Specificity Test of KOH String Test to Gram Stain in Order to Classify Bacteria Causes Urinary Tract Infection



By : Gede Nanda Utama

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Faculties : Fakultas Kedokteran


The gold standard in determining the type of bacteria and the morphology are Gram Staining. New method to determine the type of bacteria Gram reaction was found using the reagent Potassium Hydroxide (KOH) called KOH string test. The KOH string test has the advantage that only need to use only one reagent, so researchers will examine how the sensitivity and specificity of the test string KOH in determining the type of reaction gram in bacteria that cause urinary tract infections. This study is a cross-sectional study with the design of diagnostic testing of KOH String test against Gram Stain. Thirty isolate bacteria samples from urine specimen obtained from Sanglah Hospital tested with KOH string test and Gram Stain to determine the type of reaction gram bacteria. The sampling technique used is consecutive sampling. The collected data were then tabulated and included in the table then performed diagnostic test. The KOH string test sensitivity reached 92.8%, and specificity reached 100%. The KOH test string has advantages compared to Gram's staining because the process is easier, cheaper and faster time compared with Gram.

Keyword : KOH string test, Gram Stain, Sensitivity, Specificity

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