EFFECT OF INCUBATION TIME ON THE ANTIBIOFILM ACTIFITY OF SODIUM HYPOCHLORITE 1 PERCENT AND HYDROGEN PEROXIDE 3 PERCENT IN STAPHYLOCOCCUS AUREUS

SKRIPSI

Abstract

By : I Made Restu Widiana

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Faculties : Fakultas Kedokteran

Department : PS. PENDIDIKAN DOKTER

Biofilm formation is one of the main virulence factor of Staphylococcus aureus associated with healthcare associated infections (HAIs). Therefore, an effective disinfection method is needed to control biofilm formation in medical devices and reducing the incident of healthcare associated infections. This study was aimed to investigate the effect of incubation time on the antibiofilm activity of sodium hypoclorite 1% and hydrogen peroxide 3%. Antibiofilm activity is observed by measuring the optical density (OD) of biofilm residue left by treatment using tissue culture plate (TCP) method. Independent sample T-Test and one-way ANOVA was used to analyze obtained data. Significant difference (Independent Sample T-Test, P=0.002) was found between the residue left by treatment with sodium hypochlorite (0.189 0.080) and hydrogen peroxide (0.316 0.137), in which treatment with sodium hypochlorite resulted in less residue compared to hydrogen peroxide. Significant difference was found between groups of 24h, 48h, and 72h incubation time after treatment with hydrogen peroxide (One-way ANOVA, P=0.004), but no difference was found after treatment with sodium hypochlorite (One-way ANOVA, P=0.495)

Keyword : Healthcare Associated Infections, Biofilm, Incubation Time, Disinfectant

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