IDENTIFICATION OF Escherecia coli SEROTYPE O157 BACTERIA WITH SORBITOL AGAR Conkey MAC (SMAC) MEDIUM ON WATERMELON PIECES SAMPLE FROM FRUITS STREET VENDORS IN DENPASAR

SKRIPSI

Abstract

By : I Made Nugraha Gunamanta Sabudi

Email :

Faculties : Fakultas Kedokteran

Department : PS. PENDIDIKAN DOKTER

E. coli is a bacteria that can cause clinical manifestations from mild to severe diarrhea, especially E. coli serotype O157 as pathogenic bacterium that can cause bloody diarrhea. Watermelon pieces sold by street vendors is a fruit that is processed in a minimalist way and have a chance contaminated with E. coli in various ways during the cutting process, storage, till packing up to the consumer. Therefore it was important to identify the E. coli serotype O157 on watermelon pieces sold by street vendors. The aim of study to determine whether there is E. coli serotype O157 or not in watermelon pieces that sold by fruit street vendor in Denpasar. The method use is using TSB grower medium, McConkey, EMBA, SMAC and gram staining to identificate of E. coli serotype O157 on watermelon pieces samples. Result got by the study from ten samples studied was found five samples showed positive signs of E. coli, and none showed signs of E. coli O157 positive. As a conclusion there is no E. coli serotype O157 showed on watermelon pieces, but found positive bacterium E.coli in the area of Denpasar, Denpasar subdistrict of North, West and East Denpasar

Keyword : Identification, E. coli, serotype O157, Watermelon, Watermelon pieces, fruits street vendors

Download : File 1 File 2 File 3