EFFECT OF ETHANOL EXTRACT OF Aloe vera (Aloe barbadensis) LEAF LATEX ON THE GROWTH OF BACTERIA Methicillin-resistant Staphylococcus aureus (MRSA) IN VITRO



By : Felita Surya Rini

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Faculties : Fakultas Kedokteran


Infectious diseases are leading cause of death in the world. Unwise use of antibiotics result in the formation of antibiotic-resistant bacteria strains such as methicillin-resistant Staphylococcus aureus. Today, the antibiotics for MRSA is vancomycin but recently MRSA has developed resistance toward this antibiotic. Aloe vera (Aloe barbadensis) is an ornamental plant with broad biological activities including antibacterial. This study aims to determine the effect of ethanol extract of A. barbadensis leaf latex on the growth of MRSA in vitro. This study uses true experimental post-test only control group design. Subjects were MRSA. Experimental materials were ethanol extract of A. barbadensis leaf latex with concentration of 10%, 30%, 50%, 70%, and 100%. Effect of inhibition seen in the presence of inhibition zone. Differences in the inhibition zone of each concentration were tested by One Way ANOVA and Chi Square. The relationship and strength of the relationship between concentrations of the extract and diameter of inhibition zone were tested by multilevel linear regression. At concentration of >30%, the extracts were capable of producing a strong inhibition against growth of MRSA (p<0.001) with zone of inhibition mean at concentrations of 10% was 10.32+1.95 mm, 30% was 13.25+1.48 mm, 50% was 15.28+2.36 mm, 70% was 15.94+2.52 mm, and 100% was 18.02+1.03 mm. Inhibition zone diameter increased significantly along with the increased in concentration (p<0.001). The extract had a significant association with inhibition zone diameter (p<0.001) with the strength of the correlation was very strong. Antibacterial activity is due to the anthraquinone found in the leaf latex of A. barbadensis working either as bacteriostatic or bactericidal. Ethanol extract of A. barbadensis leaf latex had been proven to affect the growth of MRSA colonies even at the smallest concentration (10%) (p<0.001). However, further research is needed related to the mechanism of extraction, MIC (minimum inhibitory concentration), dosage, side effects, and toxicity of the extract before it can be applied to the community.

Keyword : MRSA, ethanol extract of A. barbadensis leaf latex, anthraquinone

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