IDENTIFICATION VIRUS AND EPIDEMIC FACTORS OF VEIN BANDING MOSAIC DISEASE ON LONG YARD BEAN PLANTS (Vigna sinensis L) in BALI

DISERTASI

Abstract

By : Trisna Agung Phabiola

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Faculties : Fakultas Pertanian

Department : S3 Ilmu Pertanian

Long yard bean (Vigna sinensis, L.) is one of the horticultural crops that widely grown in Indonesia. In the growing season 2014, planting season mosaic vein banding disease symptoms there is an appeal on the plant long yard beans dinfected virus that causes the production of beans to decrease. The purpose of this study are: (1) Determine the type of symptoms and the incidence of mosaic vein banding disease long appeal on bean plants in Bali . ( 2 ) Identify the mosaic vein banding virus that cause disease in plants chickpea appeal in Bali. ( 3 ) Study of factors the epidemic factors involved in disease progression mosaic vein banding appeal on long yard bean plants in Bali. The study was conducted through serologi test with Elisa, use technique molukuler by RT - PCR for the detection of plant viruses and continued with tracing sequence coat protein gene nucleotide to analyzed its proximity with some isolates from Gene Bank. In this study explore whether this mosaic vein banding virus can be transmitted through the mechanical transmission, through seeds, and or via insect vectors. Some weed species found around the planting of beans have the possibility as a source of inoculum in the field. The results showed that the type Potyvirus that infects long yard bean plants Bali with typical symptoms mosaic vein banding is the Bean Common Mosaic Virus (BCMV) which has a tape measure RNA of about 650 bp, and based on pilogenetika analysis of coat protein gene nucleotide sequence Bali isolate had high similarity (97.3%) with Indonesia isolates (West Java isolates). Factor Epidemic mosaic vein banding disease that varieties susceptible like varieties KPK, AURA, Pusaka Hijau, Panah Merah and seeds from farmers, the seeds that have been contaminated by the virus (seed-borne) and insect vectors that can positively transmit the virus BCMV such as Aphis craccivora, Aphis gosypii and Myzus persicae. Some types of crops and weeds mechanically inoculated with virus BCMV showed positive results on tomatoes, green beans, amaranthus tricolor and henna flowers, while weeds are positively infected with the virus BCMV Ageratum conyzaides, Ipomia triloba and Drimitia cordata.

Keyword : Long yard bean, mosaic vein banding, epidemi factor

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