THE USE OF CRUDE ANTIGEN CYSTICERCUS CELLULOSAE TO DETERMINE SEROPREVALENCE CYSTICERCOSIS IN PIGS
By : Dewa Ketut Raeyadi Purnama Stelen AR
Email : Rae_Purnama@yahoo.com
Faculties : Fakultas Kedokteran Hewan
Department : Profesi Dokter Hewan
Cysticercosis is an important parasitic zoonotic disease in animals and human. Pigs are intermediate host for this worm development and can act as transmitters of disease to humans. This research aims to use crude antigen Cysticercus cellulosae from local isolates for determine the seroprevalence of cysticercosis in pigs with Enzyme Linked Immunosorbent Assay (ELISA) method. Pork tapeworm larvae (Cysticercus cellulosae) as much as 10 grams of prepared suspension with Phosphate Buffer Solution (PBS), then Cysticercus cellulosae antigent protein protein levels were measured by Q-fluorometer. Optimization of ELISA test carried out to obtain the highest dilution of antigen, serum and conjugate. Ninety-three serum samples of swine origin Baliem Valley, Papua shipped in refrigerated boxes, then determined seroprevalence of cysticercosis with ELISA test. The results showed that the Cysticercus cellulosae antigen protein content of 876 µg/ml, the highest dilution of Cysticercus cellulosae antigen is100 µg/ml, the highest dilution of pig’s serum 1: 50, and the highest dilution of conjugate 1: 2000. Seroprevalence of cysticercosis in pigs of Baliem Valley, Papua is 59,14%. It is recommended to perform the monitoring and surveillance of swine cysticercosis on a regular basis to prevent and control this disease.
Keyword : Cysticercus cellulosae antigen, seroprevalence, cysticercosis