Used Antigen Crude Cysticercus Cellulosae Bali Isolate to ELISA Test Optimilization

SKRIPSI

Abstract

By : Inti Sari Pati Rezeki Ulina Sianturi

Email : phinsea@yahoo.com

Faculties : Fakultas Kedokteran Hewan

Department : Profesi Dokter Hewan

Cysticercosis is a parasitic disease caused by tapeworm larvae called cysticercus. Cysticercus found in pigs is Cysticercus cellulosae which is the larvae of the tapeworm Taenia solium. This research was to evaluate Taenia solium cystisercus antigen for the detection of swine cysticercosis. Taenia solium Cysticercus antigen derived from local isolates, obtained from the pig infection of Taenia solium tapeworms from Bali especially Karangasem. The research was done by ELISA (Enzime Linked Immunosorbent Assay) optimization by determining the optimal concentration of antigen, optimal dilutions of serum and optimal dilutions of conjugate. The results showed that Taenia solium Cysticercuscrude antigen (Bali isolate) are antigenic and can be used to detect swine cysticercosis with optimal concentration of antigen: 0.3125 g/ml, optimal dilutions of serum: 1:50 and optimal dilution of conjugate: 1:2000.

Keyword : Cysticercus cellulosae, crude antigen, ELISA

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